Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications. It is the original fluorescein molecule functionalized with an isothiocyanate reactive group (-N=C=S), replacing a hydrogen atom on the bottom ring of the structure. FITC is reactive towards nucleophiles including amine and sulfhydryl groups on proteins. This rabbit antibody detects the FITC only. This antibody can be coated in the plate to capture the FITC conjugated protein or antibody. Moreover, this rabbit antibody used with HRP goat anti-rabbit secondary antibody, converts fluorescent signal to DAB dyeing.
Antibodies
Application
Elisa
Application Note
For research purposes only. Not for use in humans.
Dilution
1:500 - 1:1000
Host
Rabbit
Immunogen
Synthesized peptide derived from FITC
Purity
Affinity purified
Source
Rabbit
Storage
Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.
