The first reports of atypical pneumonia in China were announced at the very end of 2019, in the city
of Wuhan, Hubei province. Its cause was identified in January 2020 as a novel β-CoV (1), named
SARS-CoV-2, which started spreading very quickly causing a global pandemic COVID-19.
Spike protein attaches the virion to the cell membrane by interacting with host receptor, initiating
the infection. Binding to human ACE2 receptor and internalization of the virus into the endosomes
of the host cell induces conformational changes in the Spike glycoprotein (2, 3). The protein forms
a trimer in vivo mediating receptor binding and membrane fusion (4). It has been demonstrated by
Florian Krammer’s group that certain mutations and the inclusion of trimerization motif can
stabilize recombinant Spike protein (5, 6).
The original Wuhan strain of the virus has become quickly replaced by its more transmissible
variant, mainly determined by a single amino acid point mutation D614G (7). A novel SARS-CoV-2
variant, B.1.617, known as Indian variant, originated in India and quickly became dominant (8,9).
Product is shipped either on dry or wet ice or frozen gel packs. Upon receipt, store at -20°C to -70°C
Antibodies
Amino Acids
16-1211
Application Note
For research purposes only. Not for use in humans.
Endotoxins
Less than 0.1 ng/µg (1 IEU/µg), as measured by LAL method.
Formulations
PBS 20% Glycerol
Grade & Purity
>90% as estimated by SDS-PAGE stained with Instant Blue Stain (Abcam).
