The first reports of atypical pneumonia in China were announced at the very end of 2019, in the city of
Wuhan, Hubei province. Its cause was identified in January 2020 as a novel β - CoV (1), named SARS CoV-2, which started spreading very quickly causing a global pandemic COVID-19.
Spike protein attaches the virion to the cell membrane by interacting with host receptor, initiating the
infection. Binding to human ACE2 receptor and internalization of the virus into the endosomes of the
host cell induces conformational changes in the Spike glycoprotein (2, 3). The protein forms a trimer in
vivo mediating receptor binding and membrane fusion (4). It has been demonstrated by Florian
Krammer’s group that certain mutations and the inclusion of trimerization motif can stabilize
recombinant Spike protein (5, 6).
The original Wuhan strain of the virus has become quickly replaced by its more transmissible variant,
mainly determined by a single amino acid point mutation D614G (7).
The B.1.1.7 variant is a strain of the coronavirus with 8 distinctive mutations (in addition to the D614G)
in the virus's spike protein and was first identified in autumn 2020 as the result of genetic sequencing
of positive COVID-19 samples by the COVID-19 Genomics UK Consortium (8).
Product is shipped either on dry ice or frozen gel packs. Upon receipt, store at -20°C to -70°C.
Antibodies
Amino Acids
16-1211
Application Note
For research purposes only. Not for use in humans
Endotoxins
Less than 0.1 ng/µg (1 IEU/µg), as measured by LAL method.
Formulations
PBS 20% Glycerol
Grade & Purity
>90% as estimated by SDS-PAGE stained with Instant Blue Stain (Expedeon).
