This Kit is a highly sensitive two-step Immunohistochemistry kit, which is suitable for the primary antibody derived from Rabbit. The kit can be used in Western blot, and IHC applications. The colour reaction principle is based on DAB reaction. Firstly, primary antibody of Rabbit origin binds to the antigen in test species. Then secondary antibody, HRP-conjugated Goat Anti-Rabbit IgG(H+L) combined with primary antibody. Next, DAB interacts with HRP, because DAB is the substrate of horseradish peroxidase (HRP). Under the catalysis of HRP, DAB produces Brown precipitation to realize signal amplification and colour development.
Antibodies
Application
Western Blot, and Immunohistochemistry Applications
Application Note
For research purposes only. Not for use in humans.
Dilution
1:1000
Form
Liquid
Host
Goat
Isotype
IgG
Purity
Affinity Purified
Shipping Condition
Transport with wet ice
Species
Rabbit
Storage
Store the secondary antibody at -20°C, DAB diluent and 50×DAB
stock solution storage at 4°C; Valid for 12 months.
