Enhanced green fluorescence protein (eGFP) is a 27 kDa protein derived from the jellyfish Aequorea Victoria, which emits green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). eGFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. eGFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize eGFP-tagged proteins within living cells without the need for chemical staining. Other applications of eGFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols.
Antibodies
Application
Western Blot
Application Note
For research purposes only. Not for use in humans.
Dilution
1:500 - 1:1000
Immunogen
KLH conjugated Synthetic peptide corresponding to Mouse eGFP
Molecular Weight
29 kDa
Positive Tissue
[WB: cells/tissues with Egfp] [IF: 293T]
Species
Human, Rat, Mouse, Tag
Storage
Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.
Synonyms
Enhanced Green Fluorescent protein, rEGFP, Recombinant EGFP, Enhanced Green Fluorescent protein, eGFP, EGFP
